2Fe-2S clusters are a class of iron-sulfur clusters that play important roles in biological processes such as electron transfer, enzyme catalysis, and sensing of environmental signals. These clusters consist of two iron atoms bound by two inorganic sulfur atoms, forming a stable, redox-active structure. The coordination of these clusters with various proteins allows them to participate in a wide range of biological functions, making them a key area of research in bioinorganic chemistry and biophysics. Studies of 2Fe-2S clusters have provided valuable insights into the mechanisms of biological redox reactions and have potential applications in drug development and biotechnology.